Insertion junction: CLIP2.007634_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.007634
Chromosome:chromosome 3
Location:4149908
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g172900 (1 of 2) PF11371 - Protein of unknown function (DUF3172) (DUF3172) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCGTCAAGTCATGGGCGACGGCACTAAACGCAGTTGCCGACACCCCGA
Internal bar code:ATCACTTCCCGTCAAATCGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2242
LEAP-Seq percent confirming:91.4894
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GCACAAAAGTCCTGCACTGG
Suggested primer 2:GTTGTCACGCTGTCCCAAAC