Insertion junction: CLIP2.007668_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.007668
Chromosome:chromosome 8
Location:2490915
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g372450 PSBQ1,OEE3, PSBQ (1 of 1) K08901 - photosystem II oxygen-evolving enhancer protein 3 (psbQ); Photosystem II Oxygen Evolution Enhancer protein 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTCAACCTAATAGCAAAGATGGCCCTCGCCTCTAAGGTTGCTACCCG
Internal bar code:GCACGGTGTAGTCTGGGGACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4236
LEAP-Seq percent confirming:98.4615
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAACATGCCGCACCTTTT
Suggested primer 2:CAAGGGAGCTTGGAAGGGAG