| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007678 |
| Chromosome: | chromosome 3 |
| Location: | 1858257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154500 | (1 of 1) PF03164 - Trafficking protein Mon1 (Mon1) | 3'UTR | |
| Cre03.g154550 | PCR1,P5CR1 | Pyrroline-5-carboxylate reductase; (1 of 1) 1.5.1.2 - Pyrroline-5-carboxylate reductase / P5CR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTGATCGACAAACCGATGAAAGGCGTCGACCGCAAGCCCAAAGGAA |
| Internal bar code: | TGGCGGTGTCAAAGTTCTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1297 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCAAGGACATGGTCACC |
| Suggested primer 2: | GCTACGGCTCTGTCACTGTT |