| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.007684 |
| Chromosome: | chromosome 2 |
| Location: | 3631251 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099850 | PDC2 | (1 of 1) PTHR11516:SF25 - PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA-3, CHLOROPLASTIC; Pyruvate dehydrogenase, E1 component, alpha subunit | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTCTCTGGCCACGTCGGAGGGGAACGCATGCAGCCCGCTGAACGCTCG |
| Internal bar code: | AGAATCGCAAGACATTCGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 825 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGTCTGCTACGCAAATC |
| Suggested primer 2: | CGATGGGCGACTTAGAGGTC |