Insertion junction: CLIP2.007688_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.007688
Chromosome:chromosome 4
Location:1695262
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g212300 PPP17 (1 of 1) PTHR13832:SF224 - PROTEIN PHOSPHATASE 2C 70; Phosphoprotein phosphatase 2C-related 5'UTR_intron
Cre04.g212350 (1 of 2) PF10013 - Uncharacterized protein conserved in bacteria (DUF2256) (DUF2256) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAACACACGCGACCGCTCGCGCTGAGGCCGGCAGCAAAATACCGCGTC
Internal bar code:ATATGACCTATTGGAAACTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1439
LEAP-Seq percent confirming:89.6552
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCACCTCGGTCATACAAA
Suggested primer 2:AGCAGGCATCCTTTGGACTC