| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.007702 |
| Chromosome: | chromosome 1 |
| Location: | 5127809 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035850 | SEC24B | (1 of 1) PTHR13803//PTHR13803:SF4 - SEC24-RELATED PROTEIN // SEC24; COP-II coat subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGGTATGGGGTCGGAGGCGACGTGTCCCGCGCGCAACGCTGTTTTAT |
| Internal bar code: | ATTGTAGCAGGGAAATTGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1713 |
| LEAP-Seq percent confirming: | 36.8421 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTGGCAGATGTCTAGG |
| Suggested primer 2: | GGCATGCCTATCATCCCTCC |