| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007708 |
| Chromosome: | chromosome 10 |
| Location: | 3389200 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443850 | (1 of 1) PTHR11851//PTHR11851:SF136 - METALLOPROTEASE // PROTEIN YHJJ | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCCGTGAGCTGTACAGCGAGAAGGCGGTGATTGAGGAGGAGCGGCGC |
| Internal bar code: | CATAAAGCTCAAGACTAGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 56.5217 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCTAATGGGGTGGGTAT |
| Suggested primer 2: | GCACTCATGTCGTGGCTTTG |