| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.007715 |
| Chromosome: | chromosome 7 |
| Location: | 1699868 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325711 | (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCCCCTGTTTGACGAGGGGCTCTCCAGGGGGGCGTAGAACCTTCCG |
| Internal bar code: | TTTGAGCTCTACGTCATGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5794 |
| LEAP-Seq percent confirming: | 67.5676 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTTGGGTGTCAGGCCAAA |
| Suggested primer 2: | ATGTGCTACTGTGCCTAGGC |