| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007715 |
| Chromosome: | chromosome 7 |
| Location: | 1699875 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325711 | (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGAGGGGAACAAAGGTGCCCATCCACACCCGGATGATACCGTAGTTCA |
| Internal bar code: | CAAGTGATCACAGCGTGTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3510 |
| LEAP-Seq percent confirming: | 79.1667 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCTACTGTGCCTAGGC |
| Suggested primer 2: | ACTTTGGGTGTCAGGCCAAA |