Insertion junction: CLIP2.007721_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.007721
Chromosome:chromosome 1
Location:805041
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g004450 CPLD42 (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517); conserved expressed membrane protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTAACCGAGGTGGGGGATAGCGCAATGTGACGTGCTGATTCGTAACCTA
Internal bar code:AGGGAATGGCTGAATAAGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1265
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTGATCGTCCTGTGAC
Suggested primer 2:AGAAATATGCACGGGCGACA