| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.007722 |
| Chromosome: | chromosome 7 |
| Location: | 901014 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318850 | HSP22B | heat shock protein 22B; (1 of 2) K13993 - HSP20 family protein (HSP20) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAAGCGTGGCAAGTGCTGTTGAGCTCTTCACGCATTACTGGGTCGCCA |
| Internal bar code: | GTCAAGGAGGGATATCTATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2666 |
| LEAP-Seq percent confirming: | 94.8718 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCGGTTTGTGCCAAAC |
| Suggested primer 2: | GGACCGGCAAGACTACACAA |