| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007723 |
| Chromosome: | chromosome 1 |
| Location: | 8033085 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g059252 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATGCCCTGGCTCTGCTGACAGGTGAGGCAGCATACTGATGCCCTGCG |
| Internal bar code: | CTTTGGTCGGTGTGATATGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2118 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCGCTTGCTGCTTTTT |
| Suggested primer 2: | AGTAGTTCTGCTGCCCACAC |