Insertion junction: CLIP2.007748_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.007748
Chromosome:chromosome 2
Location:4834256
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g109250 (1 of 1) PF13394 - 4Fe-4S single cluster domain (Fer4_14) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATGGACTGGGGTTTGGGGGGCACGGCGCATGATGGAATCGGGCAAGTG
Internal bar code:TAGGTTAGTCGGAGGGAAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2232
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCCTGCTCTGCCAGATA
Suggested primer 2:TTCATTTTGGCAGCCGTGTG