| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007748 |
| Chromosome: | chromosome 7 |
| Location: | 3425561 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335400 | EttA | Putative soluble chloroplast ABCF protein, relatated to bacterial EttA which throttles translation speed; (1 of 1) PTHR19211:SF7 - ABC TRANSPORTER-RELATED PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTATCGCGCCTCTGACCTGAGTACCACAACGAGCTCCTCCCGTACCTG |
| Internal bar code: | AGCGTTGTATTCCGATCTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCCTCTTCCCTACCCCA |
| Suggested primer 2: | AGTACAAGAGCCCGGGAGAT |