| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.007754 |
| Chromosome: | chromosome 16 |
| Location: | 1931616 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656100 | (1 of 1) PF07444 - Ycf66 protein N-terminus (Ycf66_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGCTGCTGTGGCGGCTGCGTCGGCAGGATGGGGTTGCCGTAATAG |
| Internal bar code: | CGAGTGGTGAATCCCGGAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTGTTGGCCTGCAACAG |
| Suggested primer 2: | CTGCAGCCTCCAAACAACAC |