Insertion junction: CLIP2.007766_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.007766
Chromosome:chromosome 1
Location:2606360
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g015500 CGL91 (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED; Conserved in the Green Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAACGAGAACACGCTGGAGCTGACGCTGATGGACGAGGACACGCTCAC
Internal bar code:AGTAAAAAGTCATGTCGGCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3226
LEAP-Seq percent confirming:86.9565
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCATCTGCCAATCCCCAC
Suggested primer 2:ACACGAAACGGAAGCGTACT