Insertion junction: CLIP2.007769_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.007769
Chromosome:chromosome 13
Location:4424829
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g602350 (1 of 1) K01609 - indole-3-glycerol phosphate synthase (trpC) 3'UTR
Cre13.g602400 KIN8A,KIN8-1 Kinesin motor protein; (1 of 1) K10401 - kinesin family member 18/19 (KIF18_19) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTATTATGTACACTGCGAAATACCGCCACACATGTCAGTATGCACTGCA
Internal bar code:AGCGTTTTCGTATGTTCCTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:844
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGGGACGTCTCAGCAAC
Suggested primer 2:CATCTCGAGTGATGGGGACG