| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007796 |
| Chromosome: | chromosome 12 |
| Location: | 2833874 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503350 | gene_edge/mRNA_edge/3'UTR | ||
| Cre12.g503400 | PRP38 | (1 of 1) K12849 - pre-mRNA-splicing factor 38A (PRPF38A); Pre-mRNA splicing factor, SR-related protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCTTGCCATTTACACCATCTGCTGTTATTGACGAGTGTTGCGGAACT |
| Internal bar code: | GGGACTGTTGCAGCTCTGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1308 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGTGTGCAACGAGGAA |
| Suggested primer 2: | CGCAAGTGAGTTGGTGTGTG |