Insertion junction: CLIP2.007800_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.007800
Chromosome:chromosome 9
Location:2323281
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394350 NUP155 Nucleoporin 155; (1 of 1) K14312 - nuclear pore complex protein Nup155 (NUP155, NUP170, NUP157) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCGCTAGGGTACCGTCACCGTCGCTTCAAGCTCACAATCATCCTCCAC
Internal bar code:TAAGGGGAGTTTCCGTCTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:500
LEAP-Seq percent confirming:27.2727
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGATCCTGCGCCAATCCTAT
Suggested primer 2:TCCCCAAGATCGTCTACCGT