Insertion junction: CLIP2.007801_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.007801
Chromosome:chromosome 12
Location:5486545
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g530000 (1 of 11) 2.7.11.24 - Mitogen-activated protein kinase / Stress-activated protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCTCGGTGCTACATGCGGCATCCACCATGGGCTGTTGTTCCTGCCGC
Internal bar code:CAGGACGGCAATGTGGACTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3021
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTGCGGTGTTGTCTGGAG
Suggested primer 2:ATACCCTCCGAGTTGTTGCG