Insertion junction: CLIP2.007836_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.007836
Chromosome:chromosome 12
Location:5859904
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533351 CLPB5 (1 of 3) K03695 - ATP-dependent Clp protease ATP-binding subunit ClpB (clpB); ClpB chaperone, Hsp100 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCGCATGTTTCTGATTCCACACAACCACCCCGCCTCTGGATCACTGGT
Internal bar code:CTGAACGCGTACCGATGATGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:144
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCACACGTACACACACACAC
Suggested primer 2:ACAATCTCTGCACTCACGCA