| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.007847 |
| Chromosome: | chromosome 17 |
| Location: | 5801104 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741300 | SAK1 | Regulator of acclimation to singlet oxygen | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGGCATATGGCAGCACGCCATTGCACAGCTGCTCCCACCTTCCTCGG |
| Internal bar code: | GCGAAGCGGTTGCCAGGAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2021 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGATATTGTTGCTGCTGG |
| Suggested primer 2: | ACTCCTGAGCTGTCCTTTGC |