Insertion junction: CLIP2.007884_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.007884
Chromosome:chromosome 15
Location:605743
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g636600 FAP391 (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1); Flagellar Associated Leucine Rich Repeat Protein 391 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGCCTGCCACCACCTCGCCTGCCGCCGCTGGGGCTGCTAGGGCACGCG
Internal bar code:TGTTTGCATTTAGTAGAGACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2437
LEAP-Seq percent confirming:94.8718
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGGCTGTTCTCTTCCATT
Suggested primer 2:TCCCGTGATGTGTGACTGTG