Insertion junction: CLIP2.007884_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.007884
Chromosome:chromosome 15
Location:605746
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g636600 FAP391 (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1); Flagellar Associated Leucine Rich Repeat Protein 391 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGCGTCGACGGCTGGCAAGGGAGGGGCAGGCGAGGCAGGAGCGGCGCC
Internal bar code:TTATCGATCACAGGGTGTGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1214
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCGTGATGTGTGACTGTG
Suggested primer 2:TGCGGCTGTTCTCTTCCATT