| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.007912 |
| Chromosome: | chromosome 2 |
| Location: | 8536205 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141400 | PCK1 | (1 of 1) 4.1.1.49 - Phosphoenolpyruvate carboxykinase (ATP) / Phosphopyruvate carboxylase; Phosphoenolpyruvate carboxykinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAATATAGCAGCTCTGCTGACATGCTGGCCTTGCAGGTTGCGACGGC |
| Internal bar code: | AGGGGGTTGTTGATTACTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3954 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 178 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 178 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCGGGTAAGCTCCTAG |
| Suggested primer 2: | ACATGCAAACTCTCGGCAGA |