Insertion junction: CLIP2.007928_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.007928
Chromosome:chromosome 14
Location:1195204
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g615600 SDR35 (1 of 3) IPR007751//IPR029058 - Domain of unknown function DUF676, lipase-like // Alpha/Beta hydrolase fold; Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGAGAAGCCAGGTGCGTGGTGTCAATACCCGGCACGGCGTTGACACCA
Internal bar code:CCAAATCACTCGGGTAGATGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2069
LEAP-Seq percent confirming:93.9394
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CGAACAACCAGAAAACGCGT
Suggested primer 2:CATGATCTGGTAGCCCGACC