| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.007932 |
| Chromosome: | chromosome 12 |
| Location: | 5829993 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533201 | (1 of 3) PF07452 - CHRD domain (CHRD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCACCCAGTGCACGTGCGCCATGGTGACTTTACGCAGCTTGCCGGTG |
| Internal bar code: | TGGTCGTGGTGCGAGATCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1897 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTCGGCAAATCTCTCCC |
| Suggested primer 2: | TGCTACCTCTTTGCATGCCA |