| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007940 |
| Chromosome: | chromosome 10 |
| Location: | 4016246 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447500 | KIN12-3,KIN16B,KIN16-2 | Kinesin motor protein; (1 of 1) K10392 - kinesin family member 1 (KIF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAGGTAGAACTCCTCCGCCTTGGACCAGCGCAGCTTCAGCGGCCCGC |
| Internal bar code: | GGGCTCGTCCGGAATTAACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3652 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACACGATGGAGGGCTAC |
| Suggested primer 2: | ACATGCACAGGAGGATGTGG |