| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007947 |
| Chromosome: | chromosome 1 |
| Location: | 4667910 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032250 | (1 of 1) PTHR13087:SF0 - UPF0396 PROTEIN CG6066 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTGTCGCGGCCCGGGGTCCCGCCGGCTAGCCGTTTGCGGTGCTTGCG |
| Internal bar code: | GTCGCAAAGAGAATTCGACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGGTTTGAGTGCAGGTG |
| Suggested primer 2: | GTGGCTACGTGTCTCCAACA |