Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.007960 |
Chromosome: | chromosome 7 |
Location: | 1010337 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g319600 | FAE3 | (1 of 3) K15397 - 3-ketoacyl-CoA synthase (KCS); Putative 3-keto-acyl-CoA synthase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTATGACGCTGCAGTGACGCTACAGTTGCACATTCCATGCATGAGTCC |
Internal bar code: | GTCAATCGTCTTACGTTCGTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1135 |
LEAP-Seq percent confirming: | 75.0 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCGAGCCAAATACGAACTG |
Suggested primer 2: | CTGCTACTGCTGCTGCTCA |