| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007985 |
| Chromosome: | chromosome 1 |
| Location: | 2599542 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015400 | (1 of 2) PF05542 - Protein of unknown function (DUF760) (DUF760) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCAGCGCAGCGCTCGGCCTGCAAGCCTAATGCCATTAGCCGAAGGG |
| Internal bar code: | GTGGGGTAGGTTATTAACAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4250 |
| LEAP-Seq percent confirming: | 96.2264 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACAGCCGTACTTATGCA |
| Suggested primer 2: | CGCGCATGTATGGAAACTGG |