| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008011 |
| Chromosome: | chromosome 3 |
| Location: | 7585112 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204750 | (1 of 1) PF12928 - tRNA-splicing endonuclease subunit sen54 N-term (tRNA_int_end_N2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCATCGCGGGGTCAGGCCGAATGGTCGCCCGTGGGGTTCCAGGCTT |
| Internal bar code: | GGTCCTACGCTTGGTCGGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1413 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGAGGAGGCCGTGTGAGT |
| Suggested primer 2: | CGGTACTTACACCCACCCAC |