| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008011 |
| Chromosome: | chromosome 4 |
| Location: | 3085297 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGTTCGAAGCCCCCTTCCCCCTCCCCTTACTATCTCTCACCTACTCC |
| Internal bar code: | GTGTATCGGTGAGGTTCGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4751 |
| LEAP-Seq percent confirming: | 52.1739 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCTGTACTATCTCACGC |
| Suggested primer 2: | GCAGTAAGCACATAAGCCGC |