| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.008012 |
| Chromosome: | chromosome 1 |
| Location: | 4303835 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029200 | DIV30,ESP1 | Separase, cell cycle protease; (1 of 1) 3.4.22.49 - Separase / Separin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTGTTTGTGTGTGTTGCAAGTATAGGCAGCCGTGTAGACCATGCTC |
| Internal bar code: | GGGCAAGTGCTTCGTAAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6536 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATGGCATCTGAAGTGGCG |
| Suggested primer 2: | GAAACGCAGAACCCGAAACC |