| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.008046 |
| Chromosome: | chromosome 17 |
| Location: | 6468093 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744247 | (1 of 1) PF07034 - Origin recognition complex (ORC) subunit 3 N-terminus (ORC3_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACACAGCGCAAAGTATCGACATCATAAATCATCTGTTTGTCTTTGTAG |
| Internal bar code: | CGTATCGTGGCTCGGAATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATCGCTCCCTACACTGC |
| Suggested primer 2: | AGGTGATGAAAGTGGAGGCG |