| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.008052 |
| Chromosome: | chromosome 12 |
| Location: | 6481495 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537641 | LPT1,LAT1 | (1 of 1) K13519 - lysophospholipid acyltransferase (LPT1, ALE1); Lysolipid Acyltransferase 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTTCCAGGTGCGTGCGTGCGTGGGTGGTTGTGTGTGTGTGTGTGTAT |
| Internal bar code: | CGTTGGATTAACCGCTACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1201 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGTCAGCATTTCGCCT |
| Suggested primer 2: | GCCGTTGTACCTGTGTCTCA |