| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008114 |
| Chromosome: | chromosome 8 |
| Location: | 854365 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361300 | (1 of 4) PF03567 - Sulfotransferase family (Sulfotransfer_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGTTAGTGTCCACGCCTTCGAAGCTGGCAAGGCAAACTAAGAAGGAGG |
| Internal bar code: | GCGTGTGTTACAGTGAACCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 815 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAGTGTGTGTGTTGCCA |
| Suggested primer 2: | CACTGCATTCATCGCTGCTC |