| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.008119 |
| Chromosome: | chromosome 1 |
| Location: | 6533563 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046237 | GEX33 | (1 of 1) PTHR10799:SF777 - DNA-BINDING PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATTGCCGGCCCTCACACCTATGCACATGTTCTGACCAATACCGGCTC |
| Internal bar code: | AGCGCGCCTATCGGCGTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 95 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGTGCGTGTGTTTGCA |
| Suggested primer 2: | GCCATCCATCCACTGCTCTT |