| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.008135 |
| Chromosome: | chromosome 1 |
| Location: | 7573938 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053150 | GPDH3,GPD3 | (1 of 3) K00006 - glycerol-3-phosphate dehydrogenase (NAD+) (GPD1); Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACCCAGGGCCACGATGTTCTTGAGCGTACCGCACAGCTCAGCACCCA |
| Internal bar code: | GTCTAAGGAATAGGTCAGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1859 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGCTTTGCATGGGTTCG |
| Suggested primer 2: | AACAGCGGGTACTTGCTCTC |