| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008145 |
| Chromosome: | chromosome 4 |
| Location: | 1867619 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218100 | FAP25 | (1 of 1) PF11539 - Protein of unknown function (DUF3228) (DUF3228); Flagellar Associated Protein 25 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAGGTGCGAGCGCCGCCGGCGTGACGGGCTATAATAGCAGGATAAGAC |
| Internal bar code: | GACCCGTTAGATGGTTGTCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAAGTTGGGCACGAAGAT |
| Suggested primer 2: | CCGCTGTTCACCTTCCATCT |