Insertion junction: CLIP2.008154_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.008154
Chromosome:chromosome 2
Location:2376130
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g090950 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGGCGAGACGGCGCCAGCCGATTGCTTTTTTCCGCCGCAACCCACCG
Internal bar code:CACTCAGATCCGAAGCTTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:820
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGGAATTTCAGGTCCTCA
Suggested primer 2:AGGATTCAGCGACAACGAGG