Insertion junction: CLIP2.008161_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.008161
Chromosome:chromosome 7
Location:1408532
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g323150 TEH4 (1 of 1) K17362 - acyl-coenzyme A thioesterase 13 [EC:3.1.2.-] (ACOT13); Thioesterase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCAGAGGGGGCTGGCACGGGCAGGGAGGCGGCCGGCACGGGCGTGCCA
Internal bar code:GTCAGCTCGACGATGCCTAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1868
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:67
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCAAAACGCGGTAAACA
Suggested primer 2:GCAAACCAAACTCCAGCCAG