| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008173 |
| Chromosome: | chromosome 12 |
| Location: | 5048716 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATACTAAGGCGGGGGAGAGTGGGGCGGTGGAGGAAGGCGAGGCTTTG |
| Internal bar code: | TCGGCATAGTTGCAGCCATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCCTCCACACACAGCTT |
| Suggested primer 2: | TAGTTGCCAGCACCTAAGCC |