Insertion junction: CLIP2.008182_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.008182
Chromosome:chromosome 13
Location:4913663
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g801582 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGGGGAGGGTGTGGGCAGTGCCCCAAAGTTGCCCGGCACCACCCGGTC
Internal bar code:AGATTACGGAAGGCGCTGTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1775
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTTTCGCATACGTCAAG
Suggested primer 2:TGTGACTGCGACTGTGAGAC