Insertion junction: CLIP2.008191_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.008191
Chromosome:chromosome 11
Location:1445567
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g469500 FXL6 FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCGCAACTACCGAGTTGGAGCGTCTCGCGCTATCCCTTGCTTGGTTG
Internal bar code:CACTCATGACATGTCTGCATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1091
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATAACTACATCCCGGCTGCG
Suggested primer 2:TACTGGGTAGCCGTCCATGA