Insertion junction: CLIP2.008205_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.008205
Chromosome:chromosome 6
Location:8122255
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g305650 PAO6,Tic55-like4,TIC55-4 Pheophorbide a oxygenase-related protein; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGCGGCGGCACGTGGCGGGCCTTCCGTGACGCCTGCCCGCACCGCCTG
Internal bar code:GCAGACCGTGGTGTCAAAAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1239
LEAP-Seq percent confirming:96.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGTGAAGTTGGGGATGG
Suggested primer 2:CTCTGGACACAATGGACCCC