| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.008220 |
| Chromosome: | chromosome 3 |
| Location: | 7298382 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207050 | RPL29 | Cytosolic 80S ribosomal protein L29; (1 of 1) PF01779 - Ribosomal L29e protein family (Ribosomal_L29e) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGTGCATGCTTTTTCCATTTTGCAGATGGACCCCAAGTTCCTGCGCA |
| Internal bar code: | TGTGTAGGCCGGATTAGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3234 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTTGCAAAATGGCGTTA |
| Suggested primer 2: | TTTTTGACGCACGCTTGAGG |