Insertion junction: CLIP2.008224_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.008224
Chromosome:chromosome 2
Location:2722500
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGAGACGCTCTCGACCTCCCCCCCCCCTCTGCTTGAGTGCCTGTCCCG
Internal bar code:TACGTTATCCCGCCTGTCAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3965
LEAP-Seq percent confirming:67.9245
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAAGTCCAGGGCAGACAT
Suggested primer 2:GCCATGCTTGTGAGGTTTGG