| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008226 |
| Chromosome: | chromosome 3 |
| Location: | 7289241 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207200 | (1 of 18) PF00650 - CRAL/TRIO domain (CRAL_TRIO) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTGGATCCCCTCCCTCCCAAAGCATGTCGCCCCACGATGGCGCGCCT |
| Internal bar code: | CAACATACCAGCTTTACGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3091 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACCGCTTGCTAATGAAG |
| Suggested primer 2: | GCGTCCTTTGACCCACTACA |