Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.008231 |
Chromosome: | chromosome 6 |
Location: | 321327 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g800592 | (1 of 1) PF05551 - Zinc-binding loop region of homing endonuclease (zf-His_Me_endon) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTGGACGCGGCCCCTCCCTTCCCAGACCGAGAAGCCCGAGGCGTCGC |
Internal bar code: | GGTAGATGTGGGCGAAAACATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1484 |
LEAP-Seq percent confirming: | 52.381 |
LEAP-Seq n confirming: | 11 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGGCACAAGACACAAGGC |
Suggested primer 2: | ACATGCCATCCTCGTGGTTT |